Symptoms of Parkinson’s Disease, such as tremors, are thought to be caused by low dopamine levels in the brain. Current treatments of Parkinson’s disease are primarily reactionary, aiming to replenish dopamine levels after dopamine-producing neurons in the brain have died. Without a more detailed understanding of the behavior of dopamine-producing neurons, it has been impossible to develop treatments that would prevent the destruction of these neurons in Parkinson’s patients.
Recent research provides insight into the inner workings of dopamine-producing neurons, and may lead to a new drug treatment that would proactively protect the neurons from decay. By examining the alpha-synuclein protein in yeast cells, scientists have determined that toxic levels of the protein have a detrimental effect on protein transfer within the cell. More specifically, high levels of alpha-synuclein disrupt the flow of proteins from the endoplasmic reticulum, the site of protein production in the cell, to the Golgi apparatus, the component of the cell that modifies and sorts the proteins before sending them to their final destinations within the cell. When the smooth transfer of proteins from the endoplasmic reticulum to the Golgi apparatus is interrupted, the cell dies.
With this in mind, researchers conducted a genetic screen in yeast cells in order to identify any gene that works to reverse the toxic levels of alpha-synuclein in the cell. Researchers discovered that such a gene does in fact exist, and have located the genetic counterpart in mammalian nerve cells, or neurons. This discovery has led to new hopes that drug therapy could potentially activate this gene, thereby suppressing the toxicity of alpha-synuclein in dopamine-producing neurons.
While drug therapy to suppress alpha-synuclein has been examined in yeast, fruitflies, roundworms, and cultures of rat neurons, researchers are hesitant to conclude that such therapies will prove successful on human patients. Alpha-synuclein toxicity seems to be one cause for the death of dopamine-producing neurons in Parkinson’s patients, but other causes may exist. Most scientists involved with Parkinson’s research do agree, however, that such promising early results provide a basis for further testing.
1) One function of the third paragraph of the passage is to
(A) highlight the many similarities between yeast cells and mammalian nerve cells
(B) explain in detail the methods used to conduct a genetic screen in yeast cells
(C) further explain the roles of various cellular components of yeast cells
(D) identify the genes in yeast cells and mammalian nerve cells that work to reverse the toxic levels of alpha-synuclein
(E) clarify the relevance of genetic testing in yeast cells to the search for a new treatment for Parkinson’s disease
2) It can be inferred from the passage that a yeast cell with toxic levels of alpha-synuclein will die because
(A) low levels of dopamine will disrupt the flow of proteins from the endoplasmic reticulum to the Golgi aparatus
(B) the gene that suppresses alpha-synuclein is missing or is not functioning properly in such yeast cells
(C) drug therapy has proven to be ineffective in yeast cells
(D) the normal distribution of proteins to the different cell components outside the Golgi apparatus will be affected
(E) alpha-synuclein is by nature a toxic protein
3) It can be inferred from the passage that current treatments of Parkinson’s Disease
(A) repair damaged cells by replenishing dopamine levels in the brain
(B) are ineffective in their treatment of Parkinson’s symptoms, such as tremors
(C) were developed without a complete understanding of dopamine-producing neurons
(D) will inevitably be replaced by new drug therapy to suppress alpha-synuclein toxicity
(E) were not developed through research on yeast cells
4. According to the passage, which of the following represents the chronology of a typical protein life in a healthy yeast cell?
(A) Protein is made in the endoplasmic reticulum, sent to the Golgi apparatus, processed and altered in the Golgi apparatus, and then delivered to other parts of the cell.
(B) Protein is created in the Golgi apparatus, modified and delivered to other parts of the cell, then decomposed by alpha-synuclein.
(C) Protein is produced in the endoplasmic reticulum, sent to the Golgi apparatus, and then decomposed by alpha-synuclein.
(D) Protein is produced in the Golgi apparatus, modified by the Golgi apparatus, distributed to the neuron, and then sent to the endoplasmic reticulum.
(E) Protein is produced by alpha-synuclein, transferred to the endoplasmic reticulum, sent to the Golgi apparatus for modification, and then distributed to the rest of the cell.
5. The primary purpose of the passage is toA. compare and contrast current treatments for Parkinson’s Disease
B. discuss new scientific findings and the implications of these findings for treating Parkinson’s Disease
C. explain the role of proteins in dopamine-producing neurons
D. introduce new research that supports an already existing treatment method for Parkinson’s Disease
E. question the scientific evidence used to support current treatments for Parkinson’s Disease