Given Biology and Chemistry each have >25
1) Every student not enrolled in Biology is also not enrolled in Chemistry- gives us no information on number of children enrolled either one of the courses or both hence NOT SUFFICIENT
2) Every student enrolled in chemistry is also enrolled in biology- This looks at the number if students common to both sets Chemistry and Biology.
Total students - Only Biology + Students enrolled in both + Only Chemistry + Students enrolled in None
Statement means Only Chemistry = 0 and question states that each Chemistry and Biology has>25. So since Chemistry has >25, these >25 of chemistry belong to the group containing students enrolled in both subjects. Since this is greater than 1,it answers the question stem of atleast 1. SUFFICIENT
Ans=Statement 2 alone is sufficient