(A) The groups being compared did not contain the same number of children.
Would having the same number of children change anything? I am not sure but, statistically speaking it wouldn't really explain the behavior shown by the two groups
(B) More time was spent observing the interactions of children with their mothers than with their fathers.
Would this create a bias? may be but then we would expect children in two groups to show different behavior towards their mothers
(C) Most of the researchers involved in the study were persons who had no brothers or sisters.
Would this cast bias on the experiment? not really, ı mean we are noting down the children's behavior and reporting them, this wouldn't really be affected by researchers' having no siblings
(D) The first-born children were, on the average, nearly three when their parents had second children
Correct This would weaken the argument because children who have siblings are having this experience for a very limited time, not long enough to affect their behavior, so this very group would be a wrong sample to conclude a study from
(E) The "other adults" described in the study consisted mainly of members of the research team.
This is irrelevant