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Why do we have semicolon connecting IC and DC? We have "though" in the second clause.

Sentence structure - Ozone reaches ....... ; Though, we have now realized....

Please let me know flaw in my understanding.

They are both independent clauses connected by a semi colon.
'though' is not a conjunction here but a conjunctive adverb. It explains the transition between the two ICs but the ICs are actually connected by the semi colon only.
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Ozone, a special form of oxygen that screens out harmful ultraviolet rays, reaches high concentrations twelve miles above Earth, where it has long appeared that it was immune from human influence; we have now realized, though, that emissions of industrial chlorofluorocarbons deplete the ozone layer.

Option elimination -
To clear some air so we don't waste time learning useless idiom rules that will not help on the exam day.
Immune to and Immune from are both correct. We use the "immune" to when something is unaffected by something else, such as "He seems immune to criticism." The immune form indicates protection or exemption from something, such as "Diplomats are immune from prosecution."

Appear can be used with to, without to, or even with that (though not preferred for conciseness) -
The solution appears to be effective.
The results appear promising.
The results appear as expected.
It appears that John is running late for the meeting because he hasn't arrived yet.

As VeritasPrepBrian pointed out, this is a Tense problem. Let's spend time on that.


(A) has long appeared that it was immune from - We need the past perfect "had long appeared" to indicate that the immunity to human influence was an ongoing state in the past, while the phrase "we have now realized" introduces a shift in the understanding or perfection.

(B) has long appeared to have been immune from - the same tense issue.

(C) has long appeared as being immune to - the same tense issue + "being" as a modifier.

(D) had long appeared immune to - ok

(E) had long appeared that it was immune to - "appeared immune" is more concise than "appeared that it was." GMAT prefers conciseness, provided it conveys the right meaning.
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