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Bunuel
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My 30-second approach to eliminating answer choices, I think boldface CR thoery and Function-of-a-sentence (RC) helps to navigate faster on this sort of a problem -
1. B and E can be eliminated because the unstated assumption is not a generalization (Definition of Generalization - a general statement or concept obtained by inference from specific cases)
2. A - Wrong - We don't know if the unstated assumption has been conclusively proven or not.
3. D - Wrong - Scope shift. Assumption is based on a different finding/fact that is different from the centre of the argument (It's concering people who develop resistence to penicillin without actually consuming it.

C - makes perfect sense and justifies the role of the first line stated in the passage.
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Answer ChoiceEvaluation
"(A) It is a hypothesis that is taken by the scientists to be conclusively proven by the findings described in the passage."Wrong. The assumption is background knowledge, not a hypothesis claimed proved by this study.
"(B) It is a generalization that, if true, rules out the possibility that some people who do not take penicillin develop bacteria resistant to it."Wrong. The assumption does not rule out resistance in non-users; in fact, that is the puzzle.
"(C) It is a point that, in conjunction with the fact that some patients who do not take penicillin develop penicillin-resistant bacteria, generates the problem that prompted the research described in the passage."Correct. The juxtaposition of (i) resistance occurring in penicillin users and (ii) resistance appearing in non-users is exactly what makes the latter cases puzzling and motivates the study.
"(D) It is the tentative conclusion of previous research that appears to be falsified by the scientists’ discovery of the mechanism by which bacteria become resistant to mercury poisoning."Wrong. The passage doesn’t say prior research concluded this and that it’s falsified; this answer misstates the relation.
"(E) It is a generalization assumed by the scientists to conclusively prove that the explanation of their problem case must involve reference to the genetic makeup of the penicillin-resistant bacteria."Wrong. The assumption is background; the genetic explanation is what the scientists propose, not something the assumption itself conclusively proves.
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