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Discovered in Cambodia in 2015, Cassava Mosaic Disease spread to Thailand and Vietnam by 2018 and was now estimated to be present in 10 percent of the surfaces cultivated in the region threatening millions of smallholders that cultivate cassava and generates US$4 billion in export revenue

Option Elimination -

A. spread to Thailand and Vietnam by 2018 and was now estimated to be present in 10 percent of the surfaces cultivated in the region threatening millions of smallholders that cultivate cassava and generates - we need past perfect "had spread." "That" can't be used with people who are "smallholders." "was" and "now" can't be used together. Wrong.

B. had spread to Thailand and Vietnam by 2018 and is now estimated to be present in 10 percent of the surfaces cultivated in the region, threatening millions of smallholders who cultivate cassava and generate - ok

C. spreading to Thailand and Vietnam by 2018 and is now estimated to be present in 10 percent of the surfaces cultivated in the region threatening millions of smallholders cultivating cassava and generating - no verb for "Cassava Mosaic Disease."

D. had spread to Thailand and Vietnam by 2018 and had been estimated to be present in 10 percent of the surfaces cultivated in the region, threatening millions of smallholders who cultivate cassava and generate - usage of "had been estimated to be present." no reason to use past perfect passive and doesn't agree with the present tense verbs - cultivate cassava and generate. Wrong.

E. has spread to Thailand and Vietnam by 2018 and is now estimated to be present in 10 percent of the surfaces cultivated in the region, threatening millions of smallholders who cultivate cassava and generates - present perfect is wrong.
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