Biologists studying a species of harvester ant observed that workers regularly transport small pebbles from the soil surface into the nest interior. These ants subsist primarily on seeds, but their mandibles are too weak to crack the shells of the hardest seed varieties available in their habitat. Thus, the pebbles may function as a grinding tool, helping workers process seeds that their mandibles cannot crack. Alternatively, the pebbles may serve to reinforce the walls of the nest's tunnels, which are prone to collapse in the loose sandy soil the colony inhabits.
In order to help decide between the two hypotheses for the pebbles' function, which of the following would it be most useful to know?
(A) Whether pebbles recovered from the nest interior show greater surface wear than pebbles of similar composition collected from the surrounding habitat surface
(B) Whether other harvester ant species that are known to collect pebbles for structural reinforcement also inhabit regions where hard-shelled seeds make up a large proportion of the local food supply
(C) Whether the majority of the pebbles are deposited in the brood-rearing chambers rather than in the granaries or along the load-bearing sections of the tunnels
(D) Whether the hard-shelled seeds that this species cannot crack contain significantly more nutrients than the soft-shelled seeds their mandibles can process
(E) Whether colonies of this species that nest in compact, stable soil - where the risk of tunnel collapse is not a concern - also transport pebbles into the nest interior
Option (E) provides a perfect control group by varying the environmental factor driving the need for tunnel reinforcement:
If the answer is YES (colonies in stable soil still bring in pebbles): It eliminates the tunnel reinforcement because structural reinforcement isn't necessary in stable soil. This shifts the weight toward the seed-grinding hypothesis.
If the answer is NO (colonies in stable soil do not bring in pebbles): It suggests that the behavior is directly tied to soil instability, supporting the tunnel reinforcement function and weakening the other.
Hence, this is the most useful thing to know. Ans. (E)